The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.
Inhaltsverzeichnis
A General. - I User s Instructions. - II General Lab Equipment. - III Preparation of Buffers. - IV Ammonium Sulfate Precipitation. - V Chromatographic Fractionation. - VI Centrifugation. - VII Abbreviations. - B Purification Protocols. - I Actin and Actin-Associated Proteins. - II Tubulin and Microtubule-Associated Proteins. - III Motor Proteins. - IV Intermediate Filament Proteins. - V Plasmamembrane and Organelle-Associated Proteins. - C Suppliers List.
